Abstract: Vacuum cavitation extraction technique was used to extract the alkaloids from Catharanthus roseus and the extraction yields of the main three alkaloids vinblastine，vindoline and catharanthine were used to estimate the extraction results. The effects of different solvents，pH value，acid，extraction time，solid-liquid ratio and extraction time on extraction yields were studied. The optimum parameters of extraction process by vacuum cavitation were as follows：extraction solvent was 50% methanol in sulfuric acid solution；pH value of extraction solvent was 1.5；solid-liquid ratio was 1∶10；C. roseus was extracted 3 times with each 25min by vacuum cavitation. The results showed that vacuum cavitation extraction technique was better than other extraction methods for low-concentration and high-value vinblastine with extraction yield up to 0.082‰.