Abstract: A novel thin-film composite (TFC) charged mosaic membrane (CMM) was fabricated by using interfacial polymerization (IP) techniques to deposit a thin film composite(TFC) onto a polyethersulfone (PES) porous support membrane. The poly(epichlorohydrin-amine) (PECH-amine) and 2,5-Diamino- benzene sulphonic acid (DIA) mixture solution was chosen as aqueous phase，and trimesoyl chloride (TMC) hexane solution as organic phase. The performance of CMM was optimized by studying the preparation parameters，such as the concentration of reactive monomers and the reaction time of polymerization. The orthogonal design experiment showed that the concentration of TMC had the greatest influence on the performance of CMM，IP time was the second，and the PECH-amine and DIA concentration had the least influence. The CMM properties of the optimized TFC membrane were tested by examining various salts. The experimental results showed that under 0.4MPa operating pressure，the retentions to the charged mosaic membrane prepared under the optimal conditions were low (less than 40%). Since the CMM can be permeable to salts but meanwhile not to low-molecular-weight organics，the CMM can be used in separating low-molecular-weight organics and salts mixture solution.