Abstract: Molecular imprinted polymers (MIPs) were prepared by using luteolin as the template molecule，acrylamide as the functional monomer and EGDMA as the crosslinking agent. The prepared MIPs were used as solid-phase extraction (SPE) agent to extract luteolin from peanut hull，which exhibited very high selectivity and good recognition ability to luteolin. The obtained luteolin with the MIPs-SPE column has a purity of 96.2%，which is 20 percentage higher than that with silica gel column. Furthermore，compared with silica gel column，the MIPs-SPE column can be used for many times after elution and regeneration.