Abstract: A mould was selected from rotten wood and was identified to be a penicillium sp. The strain’s conditions for enzyme production and enzyme-catalyzed temperature were tested．Then orthogonal test showed the basic fermentation conditions was：bagasse microwave-alkali pretreatment time 160s, no addition of tween80, inoculation amount 10%，culture time 5d．The fermentation conditions were further optimized by single factor tests. Saccharification rate reached 41.8% under the optimal condition．The reducing sugars contained in the fermentation broth were preliminary identified to be glucose and xylose by TLC detection. The research results suggest it is worth further researching in the utilization of bagasse resource.