Abstract: Studies were performed under atmospheric pressure in a fixed-bed microreactor and an on-line pulse reactor chromatograph to determine the effect of temperature，m(H2O)/m(C4) and residence time on producing propylene from C4 mixture. The experimental results showed that the yield of propylene increased with the increase of reaction temperature，but higher temperature resulted in obviously increasing dry gas yield and decreasing selectivity of propylene. The optimum m(H2O)/m(C4) and residence time could inhibit hydrogen transfer reaction and the yield of dry gas，and increase the yield and selectivity of propylene.