Abstract: Optically pure arylamines are important building blocks in drug synthesis and are commonly used as chiral auxiliaries and chiral resolving agents. Recent development in the enzymatic synthesis of enantiopure arylamines was reviewed in this paper，including lipase-based enantioselective acylation，transaminase-based asymmetric synthesis and kinetic resolution. Prospects of biocatalysts application in chiral amine synthesis were also discussed. Dynamic kinetic resolution might be a possible way to improve the yield of lipased-catalysed acylation，while the study of coenzyme regeneration system might reduce the cost in transaminase-catalysed reaction. Improvements in reaction system and process might also greatly enhance the efficiency of transaminase-catalysed reaction.