Abstract: Response surface methodology（RSM）was used to optimize the process of homogenized extraction of five main phenol glycosides and aglycones（target products）from the root of Acanthopanax senticosus（Rupr. Et Maxim.）Harms. According to Box-Behnken experiment design，four independent variables were selected，i.e. ethanol volume fraction，extracting time，homogenization power and liquid to solid ratio，to investigate the influence on extraction efficiency. Meanwhile，extraction rate and the purity of three target products were chosen as responses. The optimal parameters were obtained as：extraction time of 4.04min，ethanol volume fraction of 59.66%，homogenization velocity of 11 999.6 r/min，liquid to solid ratio of 7.96∶1 mL/g and 3 times. Under the optimized condition，the extraction rate and purity of three target products were found as 0.001 6% and 0.052% for syringin，0.018% and 0.68% for isofraxidin，0.035% and 1.10% for eleutheroside E，respectively.