Abstract: Jatropha Curcas oil was used as feedstock for producing biodiesel by transesterification，and adding sodium hydroxide，potassium hydroxide or sodium methoxide as catalyst respectively. The optimal conditions were obtained：molar ratio of methanol to oil 6∶1，reaction temperature 60 ℃，reaction time 80 min，mixing intensity 600 r/min，catalyst concentration for NaOH，KOH，or CH3ONa 1.2% of oil weight，The yield of biodiesel was above 97% . The physico-chemical indices of the biodiesel obtained met the quality standard ASTM of USA and DIN V of Germany. Through the preliminary analysis of economic benefits，by using non-edible Jatropha Curcas oil as raw material based on the whole industrial chain of the cultivation of Jatropha Curcas，the raw material cost is low，and the economic benefits are significant. It has also the advantage of not contending for land with grain.