Abstract: Enzyme must be treated properly for properties improvement before implementation at industrial scale，in which enzyme immobilization is the most important and conventional technique. This paper introduces the recent process in enzyme immobilization，including multipoint covalent immobilization，single enzyme nanoparticles，magnetic nanofibers，cross-linked enzyme crystal，cross-linked enzyme aggregate，chaperonin，artificial oil bodies and aptamer. New strategies to obtain immobilized enzyme with improved catalytic properties are emphasized，including stability，activity，enantioselectivity，recovery and reuse.