Abstract:

Magnetic composite microspheres of Fe₃O₄/P（AA-MMA-GMA）were prepared via emulsifier-free emulsion polymerization of acrylic acid（AA），methyl methacrylate（MMA）and glycidyl methacrylate（GMA）using 1,1-diphenylethlene as free radical control agent. The obtained Fe₃O₄/P（AA-MMA-GMA）microspheres were then used to prepare immunomagnetic microspheres via the reaction with an antibody of goat anti-rabbit IgG. The Fe₃O₄/P（AA-MMA-GMA）microspheres showed superparamagnetism behavior with particle size of 626 nm bearing epoxy groups on the surface. The immunomagnetic microspheres were obtained by reacting 0.5 mg of Fe₃O₄/P（AA-MMA-GMA）with 200 μg of goat anti-rabbit IgG in a phosphate buffer solution with pH 7.4 at 25 ℃ for 16 h，the coupling quantity for goat anti-rabbit IgG was 124 μg. The immunomagnetic microspheres can be specifically bound to rabbit anti-actin alpha.

摘要：

采用新型的无皂乳液聚合法制备磁性复合微球，即以丙烯酸（AA）、甲基丙烯酸甲酯（MMA）和甲基丙烯酸缩水甘油酯（GMA）为单体，1,1-二苯基乙烯为自由基控制剂，无皂乳液聚合制备了一种磁性复合微球Fe₃O₄/P（AA-MMA-GMA）。以所制备的Fe₃O₄/P（AA-MMA-GMA）微球为载体，通过共价偶联山羊抗兔IgG抗体，得到了一种免疫磁性复合微球。研究表明，Fe₃O₄/P（AA-MMA-GMA）微球表面带有环氧基，粒径为626 nm，具有超顺磁性。0.5 mg Fe₃O₄/P（AA-MMA-GMA）微球与200 μg山羊抗兔IgG抗体在pH=7.4的磷酸盐缓冲液中于25 ℃反应16 h，可得到一种免疫磁性复合微球，其山羊抗兔IgG的偶联量为124 μg，且该免疫磁性复合微球可特异性结合兔抗肌动蛋白α。