Abstract: The effect of redox potential regulation on the metabolites distribution in succinic acid production by anaerobic fermentation of Actinobacillus succinogenes was studied. In the serum bottle fermentation，potassium ferricyanide and dithiothreitol which did not have inhibition on fermentation，were selected as the oxidizing agent and reducing agent respectively. Potassium ferricyanide and dithiothreitol were used to control redox potential from －100 mV to －450 mV in 3 L fermenter. It was shown that the optimum redox potential or oxidoreduction potential (ORP) for cell growth and succinic acid production was －350 mV. Comparing with the fermentation without redox potential regulation，the productivity of succinic acid and the ratio of succinic acid to acetic acid enhanced from 0.75 g/(L·h) to 1.18 g/(L·h) and 2.5 to 3.9 respectively.

摘要： 对氧化还原电位调控在产琥珀酸放线杆菌厌氧发酵产丁二酸过程中的代谢产物分布的作用进行了研究。在血清瓶发酵培养过程中，筛选出对发酵过程无抑制作用的氧化剂铁氰化钾和还原剂二硫苏糖醇作为发酵体系的氧化还原电位调节剂。在3 L发酵罐上利用铁氰化钾和二硫苏糖醇调节发酵体系氧化还原电位值在－100～－450 mV，结果表明－350 mV为菌体生长和产丁二酸的最佳电位，丁二酸生产速率由0.75 g/（L·h）提高到1.18 g/（L·h），产物丁二酸与副产物乙酸的质量浓度比由2.5提高到3.9。