Abstract: Preparative-scale supercritical fluid chromatography was used to isolate solanesol from supercritical fluid extract. The chromatography conditions were as follows：ZORBAX SB-C18（250 mm×9.4 mm I.D.，5 μm）column was used as the steady phase; SC-CO2 with methanol as co-solvent was used as the mobile phase. The percent of modifier，temperature and pressure were investigated to get good resolution. In this paper，capacity factor and selectivity factor were also investigated. By chromatography separation，the concentration of solanesol reached 94.9%.

摘要： 采用ZORBAX SB-C18（250 mm×9.4 mm I.D.，5 μm）色谱柱为固定相，SC-CO2/甲醇为流动相，考察了超临界流体色谱分离提纯烟草萃取物中茄尼醇的工艺条件、流动相流速和改性剂含量对分离度的影响，以及温度和压力对茄尼醇容量因子、茄尼醇与相邻色谱峰组分分离度和选择性因子的影响。通过采用选定SFC分离工艺条件，茄尼醇产品纯度达到94.9%。