Abstract: Chitosan microspheres were prepared by the inverse phase suspension method. The chitosan was modified by reducing Schiff’s bases formed from the reaction of α-ketoglutaric acid and chitosan. The structure of microspheres was characterized by FTIR，SEM and XRD. The effects of adsorption time，pH，temperature，concentrations of albumin bovine and sodium chloride on the adsorption efficiency of albumin bovine were studied. The experimental results indicated that microspheres were insoluble in acidic and basic solutions. The modified chitosan microspheres adsorbed albumin bovine very well and at pH 5.0 adsorption reached equilibrium in 1 h. The adsorption data was fitted to the Langmuir relationship and the Lagergren second-order adsorption kinetics.

摘要： 采用反相悬浮法制备交联壳聚糖微球，再与α-酮戊二酸反应生成Schiff碱，NaBH4还原制得改性壳聚糖微球。用FTIR、SEM和XRD对其进行表征，并将之用于牛血清白蛋白的吸附研究。考察了吸附时间、溶液pH值、牛血清白蛋白的浓度、温度、NaCl含量等因素对牛血清白蛋白吸附的影响。结果表明，α-酮戊二酸改性交联壳聚糖微球不溶于酸和碱，对牛血清白蛋白具有良好的吸附性能，在pH =5.0时，吸附在1 h内可达平衡，吸附数据符合Langmuir等温方程和Lagergren二级动力学方程。