Abstract: In order to achieve high yield，high concentration and high productivity of metabolite production，a series of optimization strategies for the fermentation process were developed：(1) Nutrition and environmental conditions optimization strategy based on microorganism metrology; (2) Environmental conditions shift strategy in batch fermentation based on physiological characteristics; (3) Optimization strategies based on reaction kinetics model; (4) Optimization strategies based on metabolic flux analysis; (5) Optimization strategies based on bio-technology system. These optimization strategies were successfully applied to many metabolites production. Therefore，a simplifying，quantifying，modeling and grading principle for fermentation process optimization was summarized. This principle may provide an approach to enhancing the development of biotechnology and fermentative food.

摘要： 以高产量、高底物转化率和高生产强度为目标，综合运用微生物反应计量学、生化反应和传递动力学、生物反应器工程及代谢工程理论，开发了：(1) 基于微生物反应计量学的培养环境优化技术；(2) 基于微生物代谢特性的分阶段培养技术；(3) 基于反应动力学模型的优化技术；(4) 基于代谢通量分析的优化技术；(5) 基于系统观点的生物反应系统优化技术。将这些技术广泛应用于多种产品的发酵过程优化研究中并取得了成功。在此基础上总结出“简化、定量化、模型化和阶段化”的发酵过程优化基本原理，这一基本原理对提高我国发酵工业技术水平、促进生物食品产业的健康发展将起重要作用。