Abstract: The progress of extraction and purification of surfactin is reviewed，including precipitation，solvent extraction，chromatographic purification，ultrafiltration，adsorption，foam fractionation and liquid film separation，with emphasis on selection and integrated application of these methods. In-situ product removal (ISPR)，as one of integrated bioprocessing technologies，is an important research direction in industrial production of surfactin.

摘要： 阐述了沉淀法、萃取法、色谱法、超滤、吸附、泡沫分离法、液膜分离法在生物表面活性素（surfactin）分离纯化中的应用进展，并强调了各分离纯化方法的选择与综合应用。原位分离技术（ISPR）是将分离纯化与生产发酵过程相耦合的技术之一，代表了生物表面活性素工业规模生产的发展方向。